Melting point: 203 ℃
Boiling point: 397.76℃(rough estimate)
Refractive Index: 197 ° (C=7, H2O)
Trehalose, also known as Luluose and Mushroomose, is a non-reducing disaccharide composed of two glucose molecules.
Trehalose is a typical stress metabolite, which can form a unique protective film on the cell surface under harsh environmental conditions such as high temperature, high cold, high osmotic pressure and drying water loss, effectively protecting the biomolecular structure from being damaged, thereby maintaining Life processes and biological characteristics of living organisms.
1. Food industry
In the food industry, trehalose is currently being developed and researched for various uses considering the functions and properties of trehalose, such as non-reduction, moisture retention, freezing resistance and drying resistance, high-quality sweetness, and energy source. Trehalose products can be applied to a variety of foods and seasonings, etc., which can greatly improve the quality of food and increase the variety of food colors, and promote the further development of the food industry.
2. Pharmaceutical industry
Trehalose can be used as a stabilizer for reagent drugs and diagnostic drugs in the pharmaceutical industry. At present, various applications are being discussed based on the functions and characteristics of trehalose, such as non-reduction, stability, protection of biological macromolecules, and energy supply. Antibodies such as vaccines, blood globulins, viruses and other biologically active substances are dried with trehalose without freezing, and they can be recovered after rehydration. Trehalose replaces plasma as a biological product and stabilizer, which can not only be stored at room temperature, but also prevent pollution, thereby ensuring the preservation, transportation and safety of biological products.
Because trehalose has strong moisturizing effect and various physiological functions such as sunscreen and UV protection, it can be added to lotions, masks, essences, facial cleansers as a moisturizing agent, protective agent, etc., and can also be used as lipstick and oral cleaner. , sweeteners and quality improvers such as oral fragrances. Anhydrous trehalose can also be used in cosmetics as a dehydrating agent for phospholipids and enzymes, and its fatty acid derivatives are also excellent surfactants.
4. Crop breeding
Use biotechnology to introduce trehalose synthase gene into crops and express them in crops, construct transgenic plants that produce trehalose, cultivate new varieties of transgenic plants with resistance to freezing and drought, improve the cold resistance and drought resistance of crops, and can make them Appears fresh after harvesting and retains its original flavor and texture.
Trehalose can also be used for seed preservation, etc. After using trehalose, it can effectively maintain water molecules in seeds and rhizomes of seedlings, which is conducive to crop sowing, and has a high survival rate. At the same time, it protects crops from frostbite due to cold. Reducing production costs, especially the impact of the cold and dry climate in the north on agriculture, is of great significance.
Fermentation method: Due to the vigorous growth, rapid reproduction and easy cultivation of microorganisms, the use of submerged fermentation of trehalose has inherent advantages. Using trehalose-producing yeast and bacteria as starting strains, high-yielding trehalose-producing strains are selected and fermented to produce trehalose. Then adopt the method of effective separation and extraction to extract and refine.
Enzymatic synthesis method: The enzymatic synthesis method uses microbial fermentation to produce and extract relevant trehalose synthases, and uses various carbohydrates as substrates to synthesize trehalose. The biggest disadvantage of enzymes is their fragility and easy inactivation. In actual production, related synthetic enzymes are used for trehalose production. Considering the cost of maintaining enzyme activity and enzyme preparations, enzyme immobilization or cell immobilization technology can be used to protect the enzyme. kind.