Melting point: 177°C
Density: 1.041 g/mL at 25℃
Refractive index: n20/D 1.367
Storage conditions: 2-8°C
Solubility: H2O: 50 mg/mL
Acidity coefficient (pKa): 7.66(at 25℃)
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Tris(2-carboxyethyl)phosphine hydrochloride (TCEP hydrochloride) is a new type of reducing agent and deprotecting agent for sulfhydryl DNA. Sulfhydryl DNA terminal sulfur atoms tend to form dimers in solution, especially in the presence of oxygen. This dimerization greatly reduces the efficiency of some coupling reaction experiments (such as the immobilization of DNA in a biosensor); and adding tris(2-carboxyethyl)phosphine hydrochloride (TCEP hydrochloride) to the DNA solution ), which can be removed after a period of reaction to reduce DNA dimerization.
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