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Dithiothreitol is a common reducing agent, also called DTT, which has a strong reducibility. Its reducibility is largely due to the conformational stability of the six membered ring (containing disulfide bond) in its oxidation state. The reduction force is affected by pH value, and can only play a reduction role when pH value is greater than 7. This is because only the deprotonized thiol anion (- S -) has the reaction activity, while mercaptan (- SH) has no activity, while mercaptol PKA is generally ~ 8.3.
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